FIOCRUZ | Bartonella spp. in sand flies (Diptera, Psychodidae, Phlebotominae) from Brazil
Fundação Oswaldo Cruz
Description
Bartonella spp. was screened in 634 phlebotomine sand flies specimens, belonging to 44 different sand fly species, sampled between 2017-2021 in North and Northeastern Brazil. Detection by quantitative real-time polymerase chain reaction (qPCR) targeting the 16S-23S ITS intergenic region, found 8.7% (55/634) of the specimens positive for Bartonella. Brazilian sand flies do harbour Bartonella and might be involved the epidemiological cycle of these agents.
Geographic Description
North and Northeast of Brazil.
Data quality
Sand flies were identified according to the classification of Galati (2018).
Rangel EF, Shaw JJ, editors. Brazilian Sand Flies [Internet]. Cham: Springer International Publishing; 2018 [cited 2023 Jan 21]. Available from: http://link.springer.com/10.1007/978-3-319-75544-1
Methods
Sand flies were collected using wither CDC light traps or Shannon traps to assess anthropophily.
DNA was extracted from dissected sand flies, only thorax and abdomen as heads and three last abdominal segments were used for morphological identification using the TRIzolTM (Invitrogen®, Thermo Fisher Scientific, https://www.thermofisher.com/), following the manufacturer instructions. The presence of potential PCR inhibitors was assessed using a conventional PCR based on the cytochrome c oxidase subunit-1 (cox-1) endogenous gene for invertebrates.
Citation
Lee D A B, Shimabukuro P H F, Brilhante A F, Arantes P V C, Franco G S S E O, Machado R Z, Maggi R G, Breitschwerdt E B, André M R (2024). FIOCRUZ | Bartonella spp. in sand flies (Diptera, Psychodidae, Phlebotominae) from Brazil. Version 1.1. FIOCRUZ - Oswaldo Cruz Foundation. Occurrence dataset. https://ipt.sibbr.gov.br/sibbr/resource?r=bartonella_detection_sf&v=1.1
Rights
This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
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